Nicotiana fusion peptide — 28-day skin-appearance study

Wrinkle count, volume, and depth

wrinkle count 16% lower, volume 11% lower, and depth 14% lower

In a 20-woman, placebo-controlled split-face study of Nicotiana Benthamiana Hexapeptide-40 sh-Polypeptide-76, the ingredient manufacturer used a preparation containing 2% material, applied twice daily. At day 28, it reported 16% lower wrinkle count, 11% lower wrinkle volume and 14% lower wrinkle depth across the full study group. Within the separate 85% responder group, wrinkle depth was 19.4% lower on average.

When it was measured

day 28

Firmness and elasticity

firmness 11% higher and elasticity 6% higher

For Nicotiana Benthamiana Hexapeptide-40 sh-Polypeptide-76, the ingredient manufacturer reports whole-cohort firmness 11% higher and elasticity 6% higher at day 28 in the 20-woman placebo-controlled split-face study using 2% material twice daily; the 95% responder subsets averaged 12.6% and 6.1% higher.

When it was measured

day 28

Pore diameter

whole-cohort pore diameter 11.6% lower

For Nicotiana Benthamiana Hexapeptide-40 sh-Polypeptide-76, the ingredient manufacturer reports whole-cohort pore diameter 11.6% lower at day 28 in the 20-woman placebo-controlled split-face study; the 80% responder subset averaged 16.2% lower, with improvement up to 44%.

When it was measured

day 28

Radiance

whole-cohort radiance 12.5% higher

For Nicotiana Benthamiana Hexapeptide-40 sh-Polypeptide-76, the ingredient manufacturer reports whole-cohort radiance 12.5% higher at day 28 in the 20-woman placebo-controlled split-face study; the 90% responder subset averaged 14.4% higher, with improvement up to 42%.

When it was measured

day 28

Hydration and transepidermal water loss

hydration 12.4% higher and transepidermal water loss 9.5% lower

For Nicotiana Benthamiana Hexapeptide-40 sh-Polypeptide-76, the ingredient manufacturer reports whole-cohort hydration 12.4% higher and transepidermal water loss 9.5% lower at day 28 in the 20-woman placebo-controlled split-face study; hydration had 100% responders, while the 90% water-loss responder subset averaged 11.2% lower.

When it was measured

day 28

Skin roughness

whole-cohort skin roughness 6.9% lower

For Nicotiana Benthamiana Hexapeptide-40 sh-Polypeptide-76, the ingredient manufacturer reports whole-cohort skin roughness 6.9% lower at day 28 in the 20-woman placebo-controlled split-face study; the 75% responder subset averaged 12.6% lower, with improvement up to 26%.

When it was measured

day 28

Skin thickness

skin thickness 8.0% higher among the 85% responder subset

For Nicotiana Benthamiana Hexapeptide-40 sh-Polypeptide-76, the ingredient manufacturer reports skin thickness 8.0% higher among the 85% responder subset at day 28 in the 20-woman placebo-controlled split-face ultrasound study, with improvement up to 16%.

When it was measured

day 28

Epidermal-lipid and barrier-associated gene expression

increased expression of selected genes associated with epidermal lipids and barrier function

For Nicotiana Benthamiana Hexapeptide-40 sh-Polypeptide-76, the ingredient manufacturer reports increased expression of selected genes associated with epidermal lipids and barrier function after 24 and 48 hours in primary human keratinocytes exposed to 20 ng/mL active, stated as 2% material.

When it was measured

24 hours; 48 hours

Collagen I

dose-responsive collagen I

For Nicotiana Benthamiana Hexapeptide-40 sh-Polypeptide-76, the ingredient manufacturer reports dose-responsive collagen I after 48 hours in adult human dermal fibroblasts and describes the highest tested condition as four times its retinol reference; this is a bench protein endpoint.

When it was measured

48 hours

Epidermal thickness and lipid staining

qualitative increases in epidermal thickness and lipid staining

For Nicotiana Benthamiana Hexapeptide-40 sh-Polypeptide-76, the ingredient manufacturer reports qualitative increases in epidermal thickness and lipid staining at day 8 in an exploratory human-skin explant from one 52-year-old donor treated with a cream containing 1.5% material.

When it was measured

day 8

Nicotiana fusion peptide — 28-day skin-appearance study

The current US formulas of Evening Moisturizer - Dry and Evening Moisturizer - Normal to Dry contain Nicotiana Benthamiana Hexapeptide-40 sh-Polypeptide-76, a plant-produced fusion peptide. Its manufacturer reports findings on skin appearance and the moisture barrier from a 28-day split-face study of the ingredient.